S. N. Nekoval’, A. V. Zakharchenko, A. E. Sadovaya, A. K. Churikova, I. S. Fedoryanskaya
Federal Scientific Center for Biological Plant Protection, Krasnodar, p/o 39, 350039, Russian Federation
Abstract. The purpose of the studies was to identify new sources of tomato resistance to Alternaria alternata strains among mutant lines of the tomato genetic collection. Seven strains of the fungus A. alternata were isolated from naturally infected plants selected in five agroclimatic zones of the Krasnodar Territory. Resistance of 15 mutant tomato lines was evaluated in 2018-19 under field and laboratory conditions in the city of Krasnodar. In the field, the plants were treated with a spore suspension of A. alternata strain. The planting scheme was 1.5x0.75 m with a randomized placement; in total, the replication was three times, 10 plants each. The greatest resistance was shown by line 568; the intensity of the development of the disease on average over two years of the study was 0.5%. Lines 500, 547, 685, 809, 51, 322, 506, and 873 demonstrated relatively high and medium resistance with an intensity of pathogen development of 3.5–24.5%. Under laboratory conditions, two drops of inoculum of each strain were applied to the separated leaf lobe. The concentration was 5x10E3 conidia/mL. The radius of the lesion, rate of spot enlargement, and degree of leaf lesion were measured from the 4th day of incubation for 5 days. We did not find any samples completely resistant to all seven strains studied. The greatest resistance was shown by lines 500, 568, and 685 – the leaves were affected by the strains on average by 2.86 to 7.94%. Line 685 showed resistance to strains 6, 11, 12, and 13; line 568 showed resistance to strains 7 and 12; line 500 showed resistance to strain 13. The correlation between the results of the field and laboratory assessments was medium and close (r = 0.63–0.72, p was less than 0.01). Lines 500, 568, and 685, which showed high resistance under field and laboratory conditions, were recognized as promising for involvement in the breeding process.
Keywords: tomato (Solanum lycopersicum L.); early blight (Alternaria alternata); resistance; breeding; mutant lines.
Author Details: S. N. Nekoval’, Cand. Sc. (Biol.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); A. V. Zakharchenko, research fellow; A. E. Sadovaya, junior research fellow; A. K. Churikova, junior research fellow; I. S. Fedoryanskaya, junior research fellow.
For citation: Nekoval’ SN, Zakharchenko AV, Sadovaya AE, et al. [Identification of new sources of tomato resistance to A. alternata strains]. Dostizheniya nauki i tekhniki APK. 2021;35(9):31-6. Russian. doi: 10.53859/02352451_2021_35_9_31.