M. V. Marchenok1,2, A. B. Podvolotskaya1,2, E. S. Fishchenko1,2
1Arnika, ul. Eniseiskaya, 23b, Vladivostok, Primorskii krai, 690039, Russian Federation
2Far Eastern Federal University, ul. Sukhanova, 8, Vladivostok, Primorskii krai, 690091, Russian Federation
Abstract. The purpose of the study was to find new promising microbial producers of vitamin B12 for its synthesis. We screened eight “wild” strains of microorganisms isolated from crop production as possible producers of cobalamins: 1 – Pseudomonas aeruginosa, 1 – Bacillus subtilis, 2 – Bacillus circulans, 3 – Pseudomonas fluorescens, 1 – Pseudomonas denitrificans. At the first stage, they were assessed by the accumulation of biomass under the same cultivation conditions. Strains B. subtilis B1 and P. denitrificans P5 had an advantage in terms of this indicator under aerobic conditions: the yield of dry biomass averaged 11.2 g/L and 12.6 g/L, respectively. At the second stage, the dynamics of bacterial biomass accumulation was studied in terms of optical density, depending on the sources of carbohydrates used (sucrose, glucose, maltose). The results on media with glucose and maltose did not differ statistically: 2.52 relative units (RU) and 2.46 RU for P. denitrificans P5 and 2.50 RU and 2.49 RU for B. subtilis B1. Significantly less biomass accumulated in nutrient media with the addition of sucrose. At the third stage, after fermentation, the concentration of cobalamins in cell extracts was measured using HPLC. B1 strain of B. subtilis produced 0.037 mg/mL of the cyanocobalamin; P5 strain of P. denitrificans produced 0.042 mg/mL. Considering the technological complexity of the B. subtilis cultivation process (alternation of aerobic and anaerobic phases), it is preferable to use the P. denitrificans strain for further research.
Keywords: producer; nutrient medium; cobalamin; vitamin B12; Bacillus subtilis; Pseudomonas denitrificans.
Author Details: M. V. Marchenok, post graduate student, research bioengineer (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); A. B. Podvolotskaya, Cand. Sc. (Med.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E. S. Fishchenko, Cand. Sc. (Tech.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Marchenok MV, Podvolotskaya AB, Fishchenko ES. [Study of the possibility of using «wild» bacterial strains as possible producers of vitamin B12 (cobalamin)]. Dostizheniya nauki i tekhniki APK. 2020;34(7):109-13. Russian. doi: 10.24411/0235-2451-2020-10719.
M. V. Marchenok1,2, A. B. Podvolotskaya1,2, E. S. Fishchenko1,2
1Arnika, ul. Eniseiskaya, 23b, Vladivostok, Primorskii krai, 690039, Russian Federation
2Far Eastern Federal University, ul. Sukhanova, 8, Vladivostok, Primorskii krai, 690091, Russian Federation
Abstract. The purpose of the study was to find new promising microbial producers of vitamin B12 for its synthesis. We screened eight “wild” strains of microorganisms isolated from crop production as possible producers of cobalamins: 1 – Pseudomonas aeruginosa, 1 – Bacillus subtilis, 2 – Bacillus circulans, 3 – Pseudomonas fluorescens, 1 – Pseudomonas denitrificans. At the first stage, they were assessed by the accumulation of biomass under the same cultivation conditions. Strains B. subtilis B1 and P. denitrificans P5 had an advantage in terms of this indicator under aerobic conditions: the yield of dry biomass averaged 11.2 g/L and 12.6 g/L, respectively. At the second stage, the dynamics of bacterial biomass accumulation was studied in terms of optical density, depending on the sources of carbohydrates used (sucrose, glucose, maltose). The results on media with glucose and maltose did not differ statistically: 2.52 relative units (RU) and 2.46 RU for P. denitrificans P5 and 2.50 RU and 2.49 RU for B. subtilis B1. Significantly less biomass accumulated in nutrient media with the addition of sucrose. At the third stage, after fermentation, the concentration of cobalamins in cell extracts was measured using HPLC. B1 strain of B. subtilis produced 0.037 mg/mL of the cyanocobalamin; P5 strain of P. denitrificans produced 0.042 mg/mL. Considering the technological complexity of the B. subtilis cultivation process (alternation of aerobic and anaerobic phases), it is preferable to use the P. denitrificans strain for further research.
Keywords: producer; nutrient medium; cobalamin; vitamin B12; Bacillus subtilis; Pseudomonas denitrificans.
Author Details: M. V. Marchenok, post graduate student, research bioengineer (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); A. B. Podvolotskaya, Cand. Sc. (Med.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E. S. Fishchenko, Cand. Sc. (Tech.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Marchenok MV, Podvolotskaya AB, Fishchenko ES. [Study of the possibility of using «wild» bacterial strains as possible producers of vitamin B12 (cobalamin)]. Dostizheniya nauki i tekhniki APK. 2020;34(7):109-13. Russian. doi: 10.24411/0235-2451-2020-10719.
M. V. Marchenok1,2, A. B. Podvolotskaya1,2, E. S. Fishchenko1,2
1Arnika, ul. Eniseiskaya, 23b, Vladivostok, Primorskii krai, 690039, Russian Federation
2Far Eastern Federal University, ul. Sukhanova, 8, Vladivostok, Primorskii krai, 690091, Russian Federation
Abstract. The purpose of the study was to find new promising microbial producers of vitamin B12 for its synthesis. We screened eight “wild” strains of microorganisms isolated from crop production as possible producers of cobalamins: 1 – Pseudomonas aeruginosa, 1 – Bacillus subtilis, 2 – Bacillus circulans, 3 – Pseudomonas fluorescens, 1 – Pseudomonas denitrificans. At the first stage, they were assessed by the accumulation of biomass under the same cultivation conditions. Strains B. subtilis B1 and P. denitrificans P5 had an advantage in terms of this indicator under aerobic conditions: the yield of dry biomass averaged 11.2 g/L and 12.6 g/L, respectively. At the second stage, the dynamics of bacterial biomass accumulation was studied in terms of optical density, depending on the sources of carbohydrates used (sucrose, glucose, maltose). The results on media with glucose and maltose did not differ statistically: 2.52 relative units (RU) and 2.46 RU for P. denitrificans P5 and 2.50 RU and 2.49 RU for B. subtilis B1. Significantly less biomass accumulated in nutrient media with the addition of sucrose. At the third stage, after fermentation, the concentration of cobalamins in cell extracts was measured using HPLC. B1 strain of B. subtilis produced 0.037 mg/mL of the cyanocobalamin; P5 strain of P. denitrificans produced 0.042 mg/mL. Considering the technological complexity of the B. subtilis cultivation process (alternation of aerobic and anaerobic phases), it is preferable to use the P. denitrificans strain for further research.
Keywords: producer; nutrient medium; cobalamin; vitamin B12; Bacillus subtilis; Pseudomonas denitrificans.
Author Details: M. V. Marchenok, post graduate student, research bioengineer (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); A. B. Podvolotskaya, Cand. Sc. (Med.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E. S. Fishchenko, Cand. Sc. (Tech.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Marchenok MV, Podvolotskaya AB, Fishchenko ES. [Study of the possibility of using «wild» bacterial strains as possible producers of vitamin B12 (cobalamin)]. Dostizheniya nauki i tekhniki APK. 2020;34(7):109-13. Russian. doi: 10.24411/0235-2451-2020-10719.
M. V. Marchenok1,2, A. B. Podvolotskaya1,2, E. S. Fishchenko1,2
1Arnika, ul. Eniseiskaya, 23b, Vladivostok, Primorskii krai, 690039, Russian Federation
2Far Eastern Federal University, ul. Sukhanova, 8, Vladivostok, Primorskii krai, 690091, Russian Federation
Abstract. The purpose of the study was to find new promising microbial producers of vitamin B12 for its synthesis. We screened eight “wild” strains of microorganisms isolated from crop production as possible producers of cobalamins: 1 – Pseudomonas aeruginosa, 1 – Bacillus subtilis, 2 – Bacillus circulans, 3 – Pseudomonas fluorescens, 1 – Pseudomonas denitrificans. At the first stage, they were assessed by the accumulation of biomass under the same cultivation conditions. Strains B. subtilis B1 and P. denitrificans P5 had an advantage in terms of this indicator under aerobic conditions: the yield of dry biomass averaged 11.2 g/L and 12.6 g/L, respectively. At the second stage, the dynamics of bacterial biomass accumulation was studied in terms of optical density, depending on the sources of carbohydrates used (sucrose, glucose, maltose). The results on media with glucose and maltose did not differ statistically: 2.52 relative units (RU) and 2.46 RU for P. denitrificans P5 and 2.50 RU and 2.49 RU for B. subtilis B1. Significantly less biomass accumulated in nutrient media with the addition of sucrose. At the third stage, after fermentation, the concentration of cobalamins in cell extracts was measured using HPLC. B1 strain of B. subtilis produced 0.037 mg/mL of the cyanocobalamin; P5 strain of P. denitrificans produced 0.042 mg/mL. Considering the technological complexity of the B. subtilis cultivation process (alternation of aerobic and anaerobic phases), it is preferable to use the P. denitrificans strain for further research.
Keywords: producer; nutrient medium; cobalamin; vitamin B12; Bacillus subtilis; Pseudomonas denitrificans.
Author Details: M. V. Marchenok, post graduate student, research bioengineer (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); A. B. Podvolotskaya, Cand. Sc. (Med.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E. S. Fishchenko, Cand. Sc. (Tech.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Marchenok MV, Podvolotskaya AB, Fishchenko ES. [Study of the possibility of using «wild» bacterial strains as possible producers of vitamin B12 (cobalamin)]. Dostizheniya nauki i tekhniki APK. 2020;34(7):109-13. Russian. doi: 10.24411/0235-2451-2020-10719.
L. I. Belyaeva, M. K. Pruzhin, A. V. Ostapenko, V. N. Labuzova, T.I. Sysoeva
Kursk Federal Agricultural Research Center, ul. Karla Marksa, 63, Kursk, 305029, Russian Federation
Abstract. The purpose of the studies was to determine the effectiveness of the glycosidase enzyme preparation Dextrasept 2 in the processing of sugar beet roots infected with mucous bacteriosis. The object of the study was freshly dug and stored roots grown in chernozem soils of the Kursk region and harvested in 2018–2019 and semi-finished sugar beet products. Laboratory experiments were conducted by modelling production processes on the basis of a second-order rotatable plan for two factors at three variation levels with the construction of correlation and regression dependencies and the calculation of a generalized optimization parameter. Bacterial infection of the root cell sap had a negative effect mainly on the technological parameters of semi-finished sugar products, which can be reduced by using glycosidase-active enzymes. The introduction of the complex preparation Dextrasept 2 containing dextranase and levanase enzymes ensured the improvement of technological parameters of semi-finished products, including an increase in the sucrose content in diffusion sap on average by 2.3% to the weight of dry matter and a decrease in the content of high molecular weight compounds by 43.4%; an increase in the purification effect of diffusion sap on average by 3.5%, a decrease in the turbidity of purified sap by 24%, which can provide an increase in the quality and yield of white sugar on average by 0.5%. The use of the preparation Dextrasept 2 at a dose from 4 kg to 8 kg per 1000 tons of sugar beet was effective in the presence of 4–8 pcs of mucous inclusions in the root cell sap. The most technologically acceptable entry points for the Dextrasept 2 preparation, taking into account the degree of bacterial infection of the sugar beet cell sap, were a belt conveyor for feeding washed root crops to the collection tank before beet slicers; a conveyor belt, on which beet chips are transferred to a diffusion unit; collection tank of pulp catcher for diffusion sap; collection tank for diffusion sap.
Keywords: enzyme preparation; glycosidase activity; sugar beet; bacterial infection; polysaccharide; semi-finished product; white sugar; technological parameters.
Author Details: L. I. Belyaeva, Cand. Sc. (Tech.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); M. K. Pruzhin, D. Sc. (Agr.), leading research fellow; A. V. Ostapenko, senior research fellow; V. N. Labuzova, senior research fellow; T.I. Sysoeva, research fellow.
For citation: Belyaeva LI, Pruzhin MK, Ostapenko AV, Labuzova VN, Sysoeva TI [Performance of glycosidase-active enzymes in the production of beet sugar]. Dostizheniya nauki i tekhniki APK. 2020; 34(7):102-8. Russian. doi: 10.24411/0235-2451-2020-10718.