UDK 332.144
Rationale for long-term projections of grain production in agriculture, Siberia, on the basis of long cycles 
I.A. Ganieva, E.A. Igmulkina
Summary. In support of long-term projections made in grain production in agriculture, Siberia, on the basis of long cycles, relevant in a rapidly growing economy that needs a constant inflow of resources, including financial. Prediction made on the basis of systematization of previously identified long cyclical swings in grain production in Siberia, determining the optimum methodology for long-term predictions, the subsequent justification of the indicators of long-term projections of grain production. Implemented innovative and inertial predictions. 
Keywords: agriculture, forecasting, agricultural cycle, a model of innovation.

UDK 635.11:631.5:631.81.001.5
Basic elements of technology of cultivation beets of table republic Komi in conditions 
S.V. Kokovkina, G.T. Shmorgunov, T.V. Hurshkajnen
Summary. Ways of preparation of seeds of a beet of a dining room are led to crop; the optimum area of a feed of plants; application of new biological product Вэрва, microcells, herbicides. 
Keywords: a beet a dining room, microcells, biological product Вэрва, Herbicides, productivity, quality.

UDK 579.26 +632.937
Bacterial strains of bacillus genus as potential basis of biopreparations for soft disease control  
M.V. Shternshis, A.A. Belyaev, T.V. Shpatova, V.I. Lutov, А.А. Leliak
Summary. Antagonistic activity of three bacterial strains (Bacillus subtilis ВКПМ В-10641, B.amyloliquefaciens ВКПМ В-10642 and B.amyloliquefaciens ВКПМ В-10643) towards pure cultures of the raspberry cane spur blight and black currant septoria causal agents preceded 70%. The results of field testingin 2008-2010 showed that strain B-10641suppressed the raspberry cane spur blight and black currant septoria development with biological efficiency 65 and 56 percent respectively. In 2010 all strains suppressed raspberry, black currant and strawberry diseases. In addition, flooding strawberry roots before seeding by bacterial suspension increased plant survival and biomass. The strains studied here could be promising biocontrol agents for disease control on soft fruit. 
Keywords: antagonistic activity, bacterial strains, biological efficiency, biopreparations, diseases of soft fruit.

UDK 579.26+632.937
The influence of biopreparation Phytop 8.67 on causing agent of turfgrass root rot 
O.N. Usova, M.V. Shternshis, A.A. Lelak
Summary. Antagonistic activity of biological preparation Phytop 8.67 based on bacteria Bacillus subtilis and B. licheniformis towards Fusarium oxysporum Shclecht. Fr., causing agent of turfgrass root rot, isolated from damaged plants of Poa pratensis L., has been estimated. It was shown that inhibitory (antagonistic) activity of the Phytop 8.67 displayed at the concentrations 106 and 107 CFU/ml. The influence of the preparation on sprouting seeds and shooting length of turfgrass Poa pratensis L. as a basic component has been noticed. At high humidity without infection lesser concentrations of Phytop 8.67 appeared to be more active. The results pointed out that the preparation is promising one for turfgrass protection against root rot. 
Keywords: turfgrass, Poa pratensis, Phytop 8.67, inhibitory activity, Fusarium oxysporum, Bacillus subtilis, Bacillus licheniformis.

UDK 634.22:631.54:581.1
The prospects of some remote hybrids using as carcass stocks for plum in siberia 
D.S. Garapov, I.A. Puchkin
Summary. In Siberia the damage of plum trees relative to asphyxiation occurs every year under a snowpack. The injury of bark and cambium takes place in the bottom stem. It is a main reason of poor plum cultivation in Siberia. The problem can be solved by grafting plum on carcass stocks tolerant to asphyxiation and frost hardy. The observation of 10 remote hybrids to be used as non-asphyxiated intercalated carcass stocks for Siberian assortment of plum is presented. Bullace 18-119 (2n = 48), Microcerasus tomentosa х_ M. erythrocarpa and hybrid myrobalan plum (4 brigade) were not enough frostproof. Cherry-plum х_ blackthorn 7-92-21 (2n = 32) and Microcerasus tomentosa х_ M. erythrocarpa were not tolerant to the damage relative to asphyxiation. Hybrids of oriental plum with Louiseania ulmifolia (3-10, 18 quarter, 61-2, 61-5), blackthorn х_ oriental plum 11-73 (2n = 24) and hybrid myrobalan plum 8-3-21 combined frost hardiness and tolerance to asphyxiation are recommended as intercalated carcass stocks. 
Keywords: plum, remote hybrids, frost hardiness, tolerance to asphyxiation, snowpack, carcass stocks.

UDK 634.11:631.542:631.559(470.32)
The effect of shoot tipping on apple tree productivity and market quality of fruit in Central chernozem zone 
A.V. Soloviev, D.Е. Fedorov, D.N. Eremeev, N.P. Sdvijkov
Summary. The cvs Snroevsky, Start, Kandil orlovsky, Kurnakovskoe, Orlovskoye poles`e, Melba, Antonovka obyknovennaya, Sinap belorusskij, Bogatyr were evaluated for the effect of shoot tipping in various terms on productivity and marketalle quality of the given cvs on 54-118 rootstock. In orchard at the onset of cropping (planting in 2005) tipping at 10 – 15 cm shoot length (the third decade of May) is considered to be the most efficient method of growth and fruit cropping control in cvs Snroevskoe, Kurnakovskoe, Kandil orlovsky resulting in yield increase from 0,6 – 0,7 up to 1,4 – 2,7 (t/na) or 129,5 – 265,3% in average for two years` period with the exception of cvs Start and Orlovscoye poles`e with the most efficient tipping at 20 – 25 cm shoot length (the second decade of June) resulting in yield in crease in the first years of cropping from 0,6 – 1,1 up to 1,8 – 3,7 (t/ha) or by 184,3 – 233,6%. In bearing orchara (planting in 2001) The tipping was the most efficient one at 10 – 15 cm shoot length (the third decade of May) resulting in yield increase from 2,3 – 2.9 up to 5,7 – 8,5 (t/ha) or by 142,5 – 188,8% in cvs Melba and Antonovka obyknovennaya (average for two years` period) In cvs Sinap belorusskij and Bogatyr tipping at 20 – 25 shoot length (the second decade of June) resulting in yield increase from 6,6 – 7,0 up to 10,4 – 15,3 or by 56,6 – 118,6%. Tipping promoted 15 – 55% increase average fruit mass. The tipping at 20 – 25 cm shoot length resulted in the highest fruit mass increase with the exception of cvs Kurnakovskoye with fruit mass increase from tipping at 10 – 15 cm shoot length ( the third decade of May). The tipping promotes yield increase due to the increment of number of fruit organ formations and fruit mass depending on cvs, the terms of tipping and orchard age. In orchard at the onset of cropping tipping is considered to be more efficient compared with bearing plantation. 
Keywords: apple, tipping, pruning, fruit quality, yield.

UDK 633.367:631.452
Role of lupine in soil fertility formation 
P.A. Chekmarev, A.I. Artyukhov, N.P. Yumashev, L.L. Yagovenko
Summary. The work summarizes data on the influence of lupine in crop rotations with different saturation level in this culture and fertilizer application on the soil adsorbing complex of grey forest soils and chernozems. Lupine cultivation, especially as a green manure, improves humus quality, content and mobility degree of available nutrients, acidity and amount of exchange cations. The content of nutrients in the topsoil in gray forest soil is supported on the initial level better when the portion of lupine in crop rotation is more than 40 %, then when this portion is 20-28 %. 
Keywords: Lupine, fertilization, the soil adsorbing complex, acidity, nitrogen, phosphorus.

UDK 631.434.1:631.445.4
Alteration of aggregate-structural condition of chernozemic soils in a geomorphological profile on polar exposure slopes 
E.V. Dubovik
Summary. Alteration of aggregate-structural condition of chernozems in a geomorphological profile on the slopes of northern and southern exposures at the inclination of 1°, 3°, 5° and on the watershed plateau as well has been studied. As a result of the studies conducted it has been established that the soil density regardless of slope location and steepness was the highest in the horizons Ap and A. The density of the horizons AB and A is characterized as optimal practically on all the variants studied. Estimation of soil structure conducted in respect to its water stability by the total amount of aggregates more than 0,25 mm obtained by wet sieving and that of aggregation index as well has shown that the water stability of soil fragments and aggregate condition are deteriorating with the depth in soil cross sections. By the results of the investigations conducted it has been established that on chernozemic soils of the northern slope with the inclination increase deterioration of the structural condition and water stability takes place which can result in intensive development of erosion processes. The soils of the watershed plateau have a good structural condition and water stability, which reveals their stability in respect to the soils of polar exposure slopes. Owing to geomorphological peculiarities of a southern slope, i.e. increase of calcium content both down through the soil profile and down the slope, improvement both of soil structural condition and its water stability takes place with the increase of the inclination degree. 
Keywords: density, slope exposure, inclination degree, aggregate-structural condition, water stability.

UDK 635.21:631.531.02
Reproduction of basic material for seed potato production: 6. Laboratory сontrol methods 
A.I. Uskov
Summary. Modern high sensitive domestic ELISA- and Quick Strip dipstick-tests for potato-affected pathogens detecting have been created: PVX, PVS, PVM, PVY, PAMV, PLRV, PVA, TRV, Clavibacter michiganensis subsp. sepedonicus (Cms), Erwinia carotovora subsp. atroseptica (Eca) син. Pectobacterium atroseptica (Patro). Sensitivity characteristics of ELISA-tests are 10-50 ng/ml for viruses detecting and 103-105 cells/ml for bacteria. Sensitivity characteristics of Quick Strip dipstick-tests are 2-8 ng/ml with time detection during 10-20 min. Correlation between ELISA-and Quick Strip dipstick-tests of PVX and PVY came up to 100 и 89% for infected and 100% for healthy plants. 
Keywords: laboratory test, immune-enzyme analysis, instant diagnosis, immunochromatography on dipsticks.

UDK: (633.3+581.5) 470.331
Influence of agroecological conditions on efficiency agrophytocenosis with participation galega orientalis of the east 
A.G. Kobzin, V.A. Tjulin, N.N. Ivanova, D.A. Vagunin
Summary. Formation of efficiency of four agrocoenosis is studied: galega orientalis east one-specific crops, galega orientalis + phleum pratense, galega orientalis + festuca pratensis, galega orientalis + phleum pratense + festuca pratensis at entering Р40К60, Р60К80. One-specific crops galega orientalis were more productive. At entering of fosforno-potash fertilizers its productivity reached 12,8 t/ hectares. The mix galega orientalis + festuca pratensis at Р60К80 was the least fruitful, it has made 10,0 t/hectares of dry weight. Higher index price activity differed galega orientalis (1-2,4), and the lowest – festuca pratensis in three-componential blend grass (0,1 – 0,2). Phleum pratense on this indicator was intermediate. The economic estimation of bean and bean -cereal herbages has shown that cultivation galega orientalis in one-specific crops with entering of the raised doses of mineral fertilizers Р60К80 has provided the highest conditionally net profit – 22833 rub/hectares at level of profitability of 249 %. Power value of a crop on exchange energy of 77044,3 Mdzh/hectares at agropower factor 8,3 and a recoupment of monetary expenses of exchange energy 8,4 Mdzh/rub. 
Keywords: productivity, inorganic nutrition, botanical structure, blend grass, galega orientalis, phleum pratense, festuca pratensis.

UDK 575.174.015.3:636.082.2
The characteristics of regional populations of breeding bulls for genes of heredity disease 
L.K. Ernst, E.A. Gladyr, P.V. Gorelov, E.A. Demidova, R.R. Shajdullin, T.H. Faizov, G.S. Sharafutdinov, F.S. Sigabatullin, N.A. Zinovieva
Summary. Based on results of DNA testing performed in laboratory of molecular genetics expertise of the All-Russian state research institute of animal breeding of the Russian academy of agricultural science the characteristic of the frequency of animals carrying the heredity defect CVM (complex vertebral malformation) and BLAD (bovine leukocyte adhesion deficiency) in breeding and young bulls was done. The average frequency of mutant alleles caused CVM and BLAD was 2.77 and 2.17 per cent, respectively. The analysis of aria distribution of bulls carrying the recessive CV allele showed that the maximal carriers’ number was in Central, North-west and Privolzhskij territory. The efficiency of DNA diagnostic for decrease of the carriers’ frequency in Holstein cattle population in Russia was shown. 
Keywords: heredity defects, complex vertebral malformation (CVM), bovine leukocyte adhesion deficiency (BLAD), molecular methods, DNA, test systems.

UDK 575.174:631.523.5
The study of microsatellite variability during creation of new syberian type of beef cattle 
E.A. Gladyr, G.M. Goncharenko, P.V. Gorelov, T.S. Goryacheva, V.A. Soloshenko, I.A. Khramtsova, N.A. Zinovieva 
Summary. The polymorphism of 13 microsatellites in Simmental and Hereford cattle breeds and their F1 crosses was studied. The analysis of allele pool of the cattle groups analyzed showed the high degree of genetic diversity. The average number of alleles per loci was 8.83±0.89, 6.92±0.71 and 7.17±0.78, respectively. In F1 animal population the higher heterozygous degree and significant heterozygous excess was observed that appearingly due to integration of allele pools of two initial breeds. The detail analysis of allele profiles of F1 population comparing to initial breeds make it possible to trace their effect on the forming of allele pool of crossbred animals. The high degree of genetic consolidation of created massive of Simmental-Hereford crosses (the assignment degree of individual to own population based on microsatellites was 90.3 per cent) as result of target selection for productive traits and type was observed. From the other hand the created massive of crossbred animals is genetically closed to the initials breeds. 95.3 per cent of the overall genetic diversity was due to within population differences and only 4.7 per cent was due to differences among populations. Thus, the system based on microsatellite analysis can be used as informative tool for evaluation of the allele introduction of initial breeds during creation of new beef type Siberian cattle. 
Keywords: microsatellites, cattle, variability, creation of new types.

UDK 575.162:636.2
The effect of dna marker genotypes on the milk productive traits of black and white cattle 
O.V. Kostunina, A.M. Bakai, G.A. Bushova, T.V. Lepechina, E.A. Gladyr
Summary. The polymorphism of kappa-casein (CSN3), alpha-lactalbumin (LALBA), diacyl-O-acyltransferase (DGAT1) and growth hormone (BGH) in black and white cattle breed was detected. The effect of complex genotypes of CSN3 / LALBA and DGAT1 / BGH on the milk productive traits of cows was studied. The significant increase of the milk yield (+973 kg, P≥0.999), the milk fat quantity (+23.8 kg, P≥0.99) and the milk protein quantity (+20.3 kg, P≥0.99) in cows with the АА / BB genotype for CSN3 / LALBA and the increase of the milk yield (+969 kg, P≥0.999) and milk protein quantity (+31.5 kg, P≥0.95) in cows with AB / AB genotype comparing to AA / AA genotype was observed. The minimal levels of the milk yield, the milk fat quantity and the milk protein quantity in cows carrying the KK / LL genotype for DGAT1 / BGH were detected. 
Keywords: DNA-markers, milk productivity, black and white cattle breed.

UDK 574.9:578.2:636.2
Genogeography study of bovine leukemia virus 
I.P. Vinogradova, E.A. Gladyr, N.V. Kovaljuk, M.V. Petropavlovskij, I.M. Donnik, L.K. Ernst, N.A. Zinovieva 
Summary. The characteristic of the test-system for genodiagnostics of bovine leukemia virus (BLV) persisted in different regions in Russia was done. The objects of our study were cows (n=100) diagnosed as BLV carriers based on results of immunodiffusion (ID) reaction. In some animals (n=18) the presence of BLV was confirmed by hematological analysis. The study was performed on black and white, Holstein and airshire cattle breeds from seven different populations of Moscow region (population MR), Chelyabinsk region (populations Ural 1 and Ural 2) and Krasnodar krai (populations South 1, South 2, South 3 and South 4). The amplification of the 444 bp fragment of env gene showed the presence of provirus DNA of BLV in 85 per cent of ID-positive animals. The subsequent restriction fragment length polymorphism (RFLP) analysis showed the persistence of two genetic variety of BLV classified according World classification to Australian and Belgian subgroups. The number of cows infected of Belgian type of BLV is varied of 36.7 to 92.3 per cent from the overall number of BLV carriers diagnosed by PCR and was in average 65.3 per cent. The presence of both BLV types analyzed (as the Australian as Belgian types) in cows having the hematological stage of disease. Thus, carrying study allowed producing the additional information about regional phylogeny of env gene fragment of bovine leukemia virus. 
Keywords: bovine leukemia virus (BVL), molecular methods, gene geography, polymorphism.

UDK 636.082.251
The evaluation of leading genealogical lines of cattle red steppe breed 
A.S. Durov
Summary. The evaluation of genealogical lines of animals of the red steppe breed, bred in the conditions of the Kulundinsky region of Altay territory are resulted. The yield of milk of the first-calf heifers belonging to lines of Banquo 19665, Dzhinasa 27, Rudne of the Ideal 9451, Erlauhta 17390 on 38% surpasses the breed standard, in a fat mass fraction on 0,46%, in dairy fat on 55%. The mature cows surpasses the breed standard in live weight on 18%, on a yield of milk on 14%, to a fat mass fraction on about 0,47%, to dairy fat on 29%. A row of authentic differences on height at withers, at rump, oblique body length between animals of genealogical lines are presented. By results of an evaluation of the daughters on dairy productivity the bulls belonging to genealogical lines of Dzhinasa 27, Erlauhta 17390 and Banquo 19665 can be more widely used in region herds, for a line of Rudne of the Ideal 9451 it is necessary to allocate effective continuers. 
Keywords: genealogical line, red steppe breed, genotype.

UDK 636.2.082:591.421
Polymorphism of a nasolabial muzzle and the conservation of the gene pool of russian cattle 
A.V. Baranov, M.V. Sirotina
Summary. Phenetic methods can be used to conserve and improve cattle populations. And the most informative among phenes of cattle are dermatophenes of nasolabial muzzle. The aim of the research was to find the differences in dermatophenes of nasolabial muzzle of some Russian cattle breeds. We are the first who studied the structure of the dermatoglyphs of nasolabial muzzle of the cattle of kostromskaya, yaroslavskaya and red gorbatovskaya breeds. It took 7 years to do this research (2005-2011). The most widespread dermatotype is “ear” (38,67 % of kostromskaya, 38,08 % of red gorbatovskaya and 33,78 % of yaroslavskaya). The feature of yaroslavskaya breed is the low frequency of occurrence of the “branch” dermatotype (3,99 %) and high frequency of occurrence of the “granular” dermatotype (13,56 %). Most of the cattle of red gorbatovskaya breed have their dermatoglyphical furrows directed “up” (38,70 %), cows of kostromskaya breed has them directed “adterminal” (31,78 %). The dermatoglyphical structure has weakly twisted furrows. More cows of kostromskaya and red gorbatovskaya breeds have symmetrical dermatoglyphs than the cows of yaroslovskaya breed. Long- and short-shaped protuberances are more native for red gorbatovskaya (92,89 and 96,65 % respectively), elliptical – for cows of yaroslavskaya breed (94,68 %). The differences in frequency of occurrence of various elements of dermatoglyphical structure of nasolabial muzzle are approved by statistics (p<0,001). Monitoring of dermatogliphical structure of nasolabial muzzle of cattle will help us to use this data in models of conservation of the gene pool of cattle breeds. 
Keywords: nasolabial muzzle, dermatoglyphs, kostromskiaya, yaroslavskaya and red gorbatovskaya breeds.

UDK 57.043:636.08
Thermal expansion of water as the factor of the cryogenic damages of spermatozoa 
V.P. Kononov, V.A. Bagirov, B.S. Yolchiev, P.M. Klenovitskyi, Sh.N. Nasibov.
Summary. It ascertained the expansion of water and ice volume at the cooling below +4°C can result a damages of the several spermatozoa structures which content different quantifies of unbound water, especially acrosome and pastacrosomal part of head. The cryoprotective effect of nonelectrolytes in medium depend on binding energy of water with ones It decreases amount unbound water in diluted semen and diminishes expansion value and the crystalloid part of ice. 
Keywords: germ cell, cryoprotective medium, acrosome, cryogenic injury.

UDK 636.2:612
Activity of metabolic enzymes during the dry period in the blood of high-producing dairy cows with different reproductive potentials 
V.B. Leybova, I.Yu. Lebedeva
Summary. The reproductive capacity of high-yield dairy cows may depend on the metabolic intensity and trend during precalving period. In the present work, a comparative investigation of activities of some metabolic enzymes involving in protein and carbohydrate metabolism – aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase and alkaline phosphatase – in the blood of dry cows of the Black Pied breed with a fraction of the Holstein blood in relation to the subsequent reproductive parameters has been performed. It has been established that, in cows with length of the calving to conception interval less than 150 days, the level of the ALT activity during the preceding dry period was 1.3 times higher than in the animals with a longer calving to conception interval and 1.5 times higher than in the infertile animals. Concurrently, a negative correlation (r = -0.38) between the ALT activity and length of the following calving to conception interval has been found. Furthermore, one month before calving, 1.6-2.1-fold rise in the AST/ALT ratio in the blood of high-producing dairy cows with a reduced fertility compared to the animals with the normal reproductive capacity has been observed. No significant differences in activities of alkaline phosphatase and lactate dehydrogenase before calving of cows with different lengths of the following calving to conception interval have been revealed. The results of the present research indicate that the ALT activity and AST/ALT ratio characterize the metabolic state of dry cows, which affects their subsequent reproductive capacity. Therefore, these biochemical blood indexes during the dry period may be used as additional criteria for diagnostics of the reproductive potential of high-producing dairy cows. 
Keywords: high-producing dairy cows, aminotransferases, metabolism, reproductive capacity.

UDK 636.2: 615.814
Decrease of еmbryonic mortality at the cows by acupuncture 
T.E. Taradaynik, G.N. Singina, N.A. Zinovieva, N.P. Taradaynik, A.V. Titov
Summary. The results of effective preventive maintenance the embryonic mortality at the cows by means of injection in a biologically active point of conditioned medium obtained by cultivation of bovine embryos in vitro and biologically active substances in microdozes are submitted. The purpose of researches consist in search of the most effective methods of preventive maintenance embryonic mortality at the cows cattle after artificial insemination. In the trial 116 cows were assign to 4 groups, the treatment 1 (n=29) was injection (not later than 10 d after artificial insemination) into biologically active point 30 (Kazeev G.V., 2000) by 0.2 ml of conditioned medium obtained by cultivation of bovine embryos in vitro after artificial insemination (AI), treatment 2 (n=29) of a GnRH injection (Surfagon, 0,2 ml) after AI into the same point; and treatment 3 (n=29) of a Ovarin injection (0,2 ml) after AI into the same point; control group (n-29) was intact. The embryo protective effect was estimated at 2 mo after insemination by rectal palpation. The number of pregnant cows in 1, 2, 3 treated and control groups was 20 (P<0.05), 18, 18 and 12 (69%, 62%, 62% and 41%) respectively. The received data show, that conditioned medium obtained by cultivation of bovine embryos in vitro, entered in acupoint 30, raises a level of pregnant оf the cows on 27,5 %, in comparison with the control. Surfagon and Ovarin in 0,2 ml render embryoprotective action at introduction in acupoint 30. 
Keywords: cattle, reproduction, еmbryonic mortality, GnRH, acupuncture, biologically active points.

UDK 636.2.082.451
The use of herbal medicines for the normalization of reproductive function in cows
Y.A. Prytkov, M.V. Varenikov
Summary. The actual use of herbal medicines for prevention and treatment interventions in domestic households has been studied. Phytomedications concern as an alternative to traditional methods of treatment and prevention, such as antibiotics and the using of complex products. The significance of herbal medicines and drugs of natural origin with immunostimulating activity in veterinary practice has been shown. Most part of the herbal remedies in their chemical nature close to organism of animals. Herbal remedies easily digest and incorporate into the biochemical processes. In contrast to synthetic drugs, phytomedications determine the intake of substances, biologically compatible with it. Review of publications of studied question has been showed a significant influence of drugs derived from plants, on the rate of clinical recovery of cows which suffer from various forms of gynecological diseases. Thus, the using phytopreparation «Era-h» with activated alkaline water and furazolidone reduces service period of cows for 17 days, preparations of nettle and shepherd's purse stimulate contraction of the muscle structures of uterine horns of cows after calving, as well as reduces the time involution of reproductive organs of cows. Cows that used oil extract clinically have been recovered 80% of cows for 4-6 days, successfully have been inseminated 87.5% of the animals and the number of days of infertility was recorded at 50,6 ± 2,3, which had exceeded the figures of the control group. The researchers were used herbal in various forms: in the form of infusions, tinctures alcohol, decoctions, oil extracts. The problem of herbal medicines and drugs of natural origin with immunostimulatory activity has been insufficiently studied, many aspects of their clinical using require further development, expanding the range of medicinal plants, rationale and implementation for veterinary practice. 
Keywords: herbal, herbal drugs, reproductive function, cows, uterine involution, endometritis.

UDK 575.86:638.123.52
Evaluation of allele pool variability under development of specialized lines of honey bee of middle russian breed 
N.A. Zinovieva, N.I. Krivtsov, M.S. Fornara, E.A. Gladyr, A.V. Borodachev, V.A. Borodachev, A.S. Berezin, V.I. Lebedev 
Summary. The study of microsatellite profiles of two honey bee lines of Priokskij type of Middle Russian breed (“Clover” selected for efficiency of pollination of red clover and “IV-ZT” selected for resistance to cold) was carried out. The heterozygote deficiency (24.5 and 10.8 per cent respectively) under relatively high degree of diversity (the average number per loci was 6.29±1.51 and 8.71±1.61, respectively) was detected. The relative genetic isolation of analyzed lines was shown: 85.7 per cent individuals of line “Clover” and 86.8 per cent individuals of line “IV-ZT” were assigned to own population based on microsatellite analysis. 
Keywords: honey bee, specialized lines, microsatellites, biodiversity.

UDK 636.4.082.4.591.39
Effect of activation conditions on development of parthenogenetic porcine embryos in vitro 
G.N. Singina, A.V. Lopukhov, N.A. Zinovieva
Summary. The positive effect of 6-dimetilaminopurin (6-DMAP) and cytochalasin B on the development of pig oocytes in vitro was shown. The cleavage rate of activated oocytes after their culture in presence of 6-DMAP and cytochalasin B for 3 hours was increased on 26.8 (p <0.001) and 23.8 (p <0.01) per cent and the blastocyst formation rate was increased on 11.0 (p <0.001) and 7.4 (p <0.05) per cent, respectively comparing to controls. 
Key word: pig, oocytes, activation, cytochalasine B, 6-dimethylaminopurine, embryo, parthenogenesis, in vitro.

UDK 574.1:636.475
Development of system for multiplex analysis of pig ISSR-markers 
T.E. Deniskova, E.A. Gladyr, E.I. Sizareva, N.A. Zinovieva
Summary. The system for analysis of pig DNA-markers on base of inter-simple sequence repeat (ISSR) was developed. The system is based on PCR amplification with fluorescent labeled primers ISSR-6 and ISSR-2 and the consequent multiplex laser detection of PCR products using DNA Analyzer. Created binary DNA matrix of ISSR profiles was analyzed using GenAlEx, v6.4 software. The study of large white, landrace and duroc breeds (n=47) showed the high degree of polymorphism of ISSR-markers: 80.8 per cent of 568 identified loci were polymorphic. The all of picks identified were observed with the frequency of 5 per cent and thus were informative. The minimal number of picks was identified in landrace breed: 199 and 195 for ISSR-6 and ISSR-2 primers, respectively. The number of identified picks in large white breed was 214 and 274 and in duroc breed – 210 and 291, respectively. The landrace breed was characterized by minimal heterozygote level – 23.3 per cent whereas the values of this characteristic in large white and duroc breeds were 26.7 and 27.0 per cent, respectively. The private picks were identified in each breed analyzed: their number in large white, landrace and duroc breeds was 15, 36 and 54, respectively. The developed system of ISSR-analysis will be used for characteristics of allele pool of pig breeds. 
Keywords: ISSR-markers, polymorphism, biodiversity, pig breeds

UDK 636.018:636.475:575.162
Breeding values of the large white boars carrying the different genotypes of ESR DNA marker
O.V. Kostjunina, N.A. Svezhentseva, N.A. Zinovieva, A.V. Shahin, A.V. Dotsev, E.I. Sizareva, K.M. Shavyrina, E.A. Gladyr, L.K. Ernst
Summary. The effect of genotype for estrogene receptor (ESR) in large white boars (n=39) on the reproductive traits of their daughters (n=625) in three parities was studied. The daughters of boars carrying BB genotype of ESR comparing to daughters of boars carrying AA and AB genotypes had larger litter size (+0.11 – +0.58 piglets), number of piglets born alive (+0.33 – +0.76 piglets) and number of piglet at weaning (+0.06 – +0.30 piglets). The significant differences for alive piglet weight in fist parity were observed: the daughters of boars carrying AA genotype has on 46.2 grams higher value of this trait comparing to daughters with BB genotype (p<0.01). The higher breeding values (EBV) of boars carrying BB genotype of ESR for number of piglets born alive, days to 100 kg and MLI complex index comparing to AA genotype (+0.23 piglets, -3.29 days, +29.2 mm2 and +5.7 points, respectively) and AB genotype (+0.25 piglets, -1.69 days, +16.7 mm2 и +2,3 points) were observed. The research results show that the genotype for ESR can be used as the additional criterion for selection of boars carrying the higher EBV for number of piglets born alive, days to 100 kg, muscle ear aria and MLI complex index including the following traits: number of piglets born alive, the litter weight at 21 day, back fat and days to 10 kg. 
Keywords: estrogene receptor, marker assisted selection, EBV, productive traits.

UDK 574.1:636.475
The characteristic of allele pool of siberian population of fast-growing meet breed using microsatellites 
V.R. Charzinova, N.A. Zinovieva, N.V. Bateneva, S.N. Lugovoj, E.A. Gladyr, G.M. Goncharenko, K.V. Zhuchaev, V.N. Maurcheva, M.A. Barsukova
Summary. The study of microsatellite profiles of Siberian branch of fast-growing pig breed (SM-1) in dinamics (populations of the year 2010 and 2011) was carryed out. It was shown, that the number of alleles in different microsatellite loci is varied of 3 to 14 and the number of effective allele is varied of 2.10 to 10.29. The maintenance of pig genetic diversity in several generations was shown; the average number of alleles per loci was 7.50±0.92 and 6.90±1.06, the number of effective alleles per loci was 4.37±0.66 and 4.04±0.79,  respectively. The introduction of new alleles in the population of the year 2011 was detected. The comparative study of microsatellite profiles of SM-1 pigs and four main pig breeds: large white (eight population, n=378, yorkshire (six population, n=127), landrace (ten population, n=467) and duroc (nine population, n=270) breeds the more closeness of SM-1 breed was carried out. The more closeness of CM-1 pigs to landrace was shown. 
Keywords: microsatellite, genetic diversity, fast-growing pig breed (SM-1).

UDK 636.4.087.76.
Influence of «Prolyser – BioR» symbiotic preparation based on lysine synthesis culture e. Coli strain VL 613 in rations of transgenic swine and their analogues on fattening 
L.K. Ernst, O.A. Artemjeva, E.N. Kolodina, E.N. Strekozova, V.A. Fomenko
Summary. For last years, the necessity of studying some alternative pathways of animal husbandry intensification using ecological principles of influencing on animal growth and development with the aim of high product yield has not failed in its actuality. First of all, such target setting is linked to the concentration of animals in great numbers on bounded areas and to the isolation from the environment, that results in shaping of closed population relations – an animal-external microbiocoenosis, an animal-internal microbiocoenosis. For the purpose of studying the efficiency of the usage of biologically active preparation on the base of lysine producer Escherichia coli VL613 in fattening hGHRF transgenic swine and their analogues, the experiment was conducted under the conditions of physiological yard of the All-Russian Research Institute for Animal Husbandry (VIZh). Consequently, average daily liveweight gains in groups I and II amounted to 760 and 780 g, respectively, for the whole fattening period, and also the slaughter product weight and viscera data and their proportions to the carcass weight were obtained. When analyzing the microflora composition, the bacteria content from the group of Escherichia coli and lactic acid microorganisms remained stable regardless of preparation feeding that indicated the high resistance of the gastrointestinal tract microbiocoenosis of experimental pigs.
Keywords: swine fattening, live weight, gastrointestinal tract microflora, colibacillus bacteria, lactic acid microorganisms, Escherichia coli VL613 «Prolizar».

UDK 575.86:636.295
Development of the test system for the microsatellite analysis of camels 
E.A. Gladyr, A.M. Zajtzev, E.P. Kudina, V.V. Kalashnikov, N.A. Zinovieva
Summary. The multiplex test-system for the simultaneous analysis of eight microsatellite loci of camels (YWLL44, YWLL08, YWLL38, LCA66, LCA19, LCA37, CMS16, and VOLP10) was developed. The informativity of created systems on example of camels of Kalmyk breed (n=32) of Camelus bactrianus L. specie was shown. The average number of alleles per loci was 6.75±1.24, the number of effective alleles per loci – 3.52±0.97. The analysis of heterozygote levels in analyzed population shows 8.7 per cent of heterozygous genotypes. The construction and analysis of genealogical tree was performed. 
Keywords: microsatellites, molecular methods, test kits, camels.

UDK 636.5.082:592 / 636.5.033
Study of the informativity of G. Gallus microsatellites for the characteristics of M. Gallopavo allele pool 
I.P. Novgorodova, V.V. Volkova, E.A. Gladyr, M.I. Selionova, E.I. Rastovarov, V.I. Fisinin, N.A. Zinovieva 
Summary. The study of two turkey breeds BIG6 and BIG10 using G. gallus microsatellites was performed. The polymorphism of seven of eight analyzed loci was shown. In six loci the presence of three and more alleles was shown that makes possible to regards they as informative for the characteristics of M. gallopavo gene pool. 
Keywords: DNA markers, microsatellite, turkey gene pool.

UDK 577.29
Species identification of poultry in livestock products by using of polymerase chain reaction method 
E.N. Konovalova, E.A. Gladyr, N.A. Zinovieva
Summary. The test-system on PCR-base allowed to carry out simultaneous identification of three avian species (Gallus gallus, Anas platyrhynchos, Meleagris gallopavo) in poultry raw was developed significantly reducing the time and reagents costs. The test-system is based on multiplex amplification of conserved region of mitochondrial gene cytochrome B by using of three species-specific primer pairs with consequent detection of PCR-products by electrophoresis method in agarose gel. It was shown the ability of using the test-system to analyze of composition of mince different species. 
Keywords: identification of species composition, molecular tools, multiplex PCR.

UDK 619:615+339:636.5
Application for cultivation Vetom 1.23 broiler chickens cross ISA F-15 
M.G. Petrash, A.N. Lukjanov, G.A. Nozdrin, , A.I. Lelyak, A.I. Vorontsova, N.V. Revkov, A.A. Leliak 
Summary. In this study has been shown the possibility of growing ISA F-15 broiler chicks with the use of antibiotic only first 5 days of age. A probiotic called Vetom 1.23 was treated instead of antibiotics from 6th day of age to the ending of chicks growing. It was established the effective therapeutic regimen is the 0.5 мl/kg for once every three days. Zootechnical parameters obtained from the experiment is not inferior to typical values of the poultry farms. 
Keywords: broilers, probiotic, strains, mortality, body weight, feed conversion ratio.

UDK 636. 5. 087. 72
Physiological indication of grows rate in the application of Vetom 13.1 
G. A. Nozdrin, T.G. Kazantseva, A. B. Ivanova, A.G. Nozdrin, O.Yu. Ledenyova, A.I. Lelyak, A.A. Lelyak 
Summary. The effect of different doses and schemes Vetom 13.1 on grows rate of geese. The influence of the drug the growth rate increases as application of the drug, and within 30-60 days after cancellation. The maximum increase was recorded in the appointment Vetom 13.1 in 50mg/kg, 1 time per days on a daily basis, then the next day, up to 2 month of age. 
Keywords: absolute grows, Vetom 13.1, geese, grows of rate, probiotic, average daily gain scheme.

UDK. 576.3/.7.086.83:612.014
Liver is immunoprivileged zone for treatment of diabetes of small domestic animals by xenotransplantation. 
I.K. Abdrakhmanov, L.A. Serezhina, M.A. Selyugin, N.M. Tuzhanskaya, Yu.A. Kuznetsov, I.F. Vilkovysky, N.B. Savenko, V.N. Mitin, L.P. Dyakonov
Summary. This article describes the experience of clinical xenotransplantation β-cells of rabbit fetuses pancreas to 5 dogs and 3 cats (8 experiments) with a diagnosis of diabetes mellitus. Cells were transplanted into the liver percutaneously through the abdominal wall into the parenchyma of the left lobe of the liver in a dose of 100 thousand cells per 1 kg of body weight using an unique non-invasive technique to enable a transplant without anesthesia. If necessary, symptomatic treatment of associated pathologies was realized. Blood glucose levels was determined 1-4 times per day after transplantation. Positive results we achieved in 6 of 8 animals, with the observed improvement in laboratory parameters: reduction of blood glucose and urine, disappearance ketoacidosis, improving the flow of diabetic complications. In 4 cases of 8 total insulin- independence over 7 months to 3 years we observed. Insulin-dependent diabetes – the disease characterized by failure to maintain normoglycemia β-cells of the pancreas. One of the treatments – transplanted islet tissue donors to patients with diabetes mellitus. The main obstacle for transplantation is acute and hyperacute transplant rejection. Methods to prevent immune rejection of the graft are the right choice immunoprivileged zone, use of «refined» pre-cultivation of β-cells and use of β-cells for a certain period of gestation in the selection of donor material. We used the liver as immunoprivileged zone, thus renounce the use of immunosuppressive drugs. 
Keywords: diabetes mellitus, xenotransplantation, β-cells of pancreas, rabbit fetus, endocrinology.

UDK 663.18:574.44
The effect of probiotic preparation based on bacteria of the genus bacillus on the growth rate of liver 
G.A. Nozdrin, S.N. Tishkov, A.G. Nozdrin, A.V. Vorontsova, A.I. Lelyak, A.A. Lelyak
Summary. The studies drugs Vetom 1.1, Vetom 4 & Vetom 14.1 increased the growth rate of mice without changing the dynamics of growth in body weight in age aspect. The severity of both depend on the applied drug & the studies doses. The maximum gain of weight of liver was recorded in the application Vetom 1.1 & Vetom 14.1 dose 3.388 gr/lb of body weight of animal. The maximum gain of weight of liver was recorded in the application Veton 4 dose 1.694 gr/lb of body weight of animal. 
Keywords: vetom, weight of animal, digestion, probiotic, symbiosis, Bacillus licheniformis, Bacillus subtillis.

UDK 631/633.15 : 63 : 061.6
Coordination of scientific investigations and innovations in fodder production of Russia 
V.M. Kosolapov, N.A. Laretin
Summary. The results of work, conducted by the leading Institute-coordinator and its coordinate network on organization the scientific investigations in 2006-2010 years, have been summarized. Basic directions of research work for 2011-2015 have been considered. A structure of system the scientific research organization in fodder production has been shown. Some problems and importance of coordination for informational supply and innovative process development in fodder production of Russia are represented. 
Keywords: fodder production, the results and prospects of development, organization and coordination of scientific work, innovative process, efficiency, scientific- research agricultural institutes and universities, experimental stations and pilot farms.